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. 2017 Dec 22;7:18045. doi: 10.1038/s41598-017-18404-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The ACER1-36 module is decreased in PP- and PN-KCs and exhibits differentiation-dependent expression. (A,B) ACER1-36 genes ranked according to their expression decrease (PP/PN-KCs, bottom margin: FC). (C) ACER1-36 expression in NN skin (GSE54456). (D) GO BP terms enriched a mong ACER1-36 genes (left margin parentheses: number of genes per term). (E) Chromosomes associated with AVER1-36 genes (right margin: Fisher’s Exact Test p-values). (F) Motifs enriched in regions 5000 bp upstream of ACER1-36 genes (white font: observed/expected motif frequency). (G) ZNF83 sequence logo. (H–J) ACER1-6 genes show decreased expression in KCs following RNAi knockdown of (H) MAF and MAFB, (I) ZNF750, and (J) STAU. Genes are ranked according to their RNAi expression change and cumulative overlap with ACER1-36 genes is shown.