Figure 1.

UII stimulates SOCE via TRPC4 channels in mouse GMCs. (A,B) Representative traces and mean data showing that UII (100 nM)-induced SOCE is attenuated by ML204 (0.5 µM; n = 7), but not, Pyr3 (1 µM; n = 4). (C,D) Western blot and mean data (n = 4 each) confirming siRNA-mediated knockdown of TRPC4 channels in mouse GMCs. (E) Mean data illustrating that siRNA-mediated knockdown of TRPC4 channels inhibits UII (100 nM)-induced SOCE in mouse GMCs [scrambled (Scram) control siRNA, n = 8; TRPC4 siRNA, n = 7]. The Ca²⁺ free solution was supplemented with EGTA (0.1 mM) to chelate Ca²⁺. To block L-type calcium channels, nimodipine (1 µM) was added to the bath solution. *P < 0.05 vs. 0 mM Ca²⁺ + 0.1 mM EGTA; ^#P < 0.05 vs. UII and Pyr3 + UII; ^$P < 0.05 vs TRPC4 siRNA.