Figure 3.

UII activates CaMKII phosphorylation in mouse GMCs. Confocal microscopy of mouse GMCs showing that: (A) Total endogenous CaMKII is immunostained in untreated and treated cells (100 nM UII for 5 min), (B) Unlike untreated cells, pCaMKII Thr²⁸⁶ immunostaining was detected in cells incubated with UII, an effect abolished by KN-93 (2 µM). Images are representative of 3-4 randomly chosen fields from 3-4 coverslips per experimental condition. Scale bar = 100 µm.