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. 2017 Dec 22;7:18050. doi: 10.1038/s41598-017-18374-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Read-depth analysis along the chromosomes formed by the fusion of two PacBio-assembled contigs. Coverage was determined by sliding window analysis (bin 200 pb) with either Illumina (in blue) or PacBio (in red) reads, along chromosomes 7, 12, 15, 22, 26, 28, 33 and 35. The sizes of the contigs are shown by lines with arrow-heads. Chromosomes 7 (panel A) and 35 (panel H) were joined by the SSPACE-standard tool. Chromosomes 12, 15, 22, 26 and 28 (panels B–F) were joined using the minimus 2 assembler. Finally, chromosome 33 (panel G) was joined by the SSPACE-LongRead tool.