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Western blot analysis for the effect of tranilast on Smad2 phosphorylation in HPMCs. Smad2 phosphorylation was observed in cells treated with TGF-β1 for 1 h, whereas pretreatment with 200 μM tranilast inhibited phosphorylation. Smad2 expression was not affected in control cells or in TGF-β1 and tranilast-treated groups
