Fig. 1.

MiR-1 expression in cultured gastric cancer (GC) cells and primary GC tissues samples, and its correlation with prognosis of GC patients a MiR-1 expression in GC cell lines compared with the immortalized gastric cell line GES-1. b Quantitative PCR levels showing reexpression of mature miR-1 in GC cell lines after 5-aza-2′-deoxycyridine (5-aza-dC) treatment. c Relative expression levels of miR-1 in primary gastric tumors and adjacent nontumor tissues (n = 90). The data represent 2^−ΔΔCt expression values. The P value was calculated by a paired test. d Kaplan–Meier curves of overall survival for all patients with miR-1-high versus miR-1-low GC tissue. e Transfection efficiency of miR-1 in GC cells by qPCR . Mean ± standard deviation of three independent experiments. WT wild type, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001