Fig. 4.

MiR-1 inhibits the expression of VEGF-A and EDN1 3′ untranlated region (UTR)-integrated luciferase reporter genes. a The target sites for miR-1 in the VEGF-A (NM_001025369.2), and EDN1 (NM_001955.4) 3′ UTR were identified with the TargetScan database. b Luciferase reporter assay using the vector encoding the full-length 3′ UTR of VEGF-A, and EDN1 3′ UTR messenger RNA. The Renilla luciferase values were used to normalize firefly luciferase values. Cont. control, Mut mutant, Wt wild type, *P < 0.05, **P < 0.01