Figure 2.

Schematic representation of the class 1 integron gene cassettes bearing the bla_IMP-4 genes in E. cloacae isolates. Genes and their directions of transcription are described as broad arrows. The gray box indicates recombination site. The primers, detailed in Table 1, for PCR mapping are depicted as narrow arrows with numbers. The red arrow of bla_IMP-4 is related to carbapenemase. The yellow arrow of qacG and aacA4 are associated with resistance to quaternary ammonium compounds and fluoroquinolone, respectively. The 5′ conserved segment (CS) of IntI1 and 3′ CS of sul1 are presented with green arrow. (A) E. cloacae YUMC2 in this study with Genbank accession no. KY884003. (B) E. cloacae EI1573 from Sydney, Australia with Genbank accession no. JX101693.1. (C) E. cloacae from Queensland, Australia reported by Sidjabat et al. (2015).