Table 7.
Designed primer sets to differentiate biovar 4 from others
| Set | F. pos (bp) | R. pos (bp) | Forward primer | Reverse primer | Tm (°C) |
|---|---|---|---|---|---|
| 8 | 281 | 435 | AGCCACGCACGACCTATATC | GCCCGAGCAATACTGATACC | 60 |
| 12 | 478 | 877 | GAAGCCGATCAGCAATTCAC | AAAGCAGGATCGCCACATAG | 60 |
| 13 | 178 | 552 | GGATTGTCGTGGCTTACGAT | GAAGGCATAGACCGTGGTTG | 60 |
| 19 | 962 | 1218 | ACGCAAGACCTTTGAAGACG | GAGCGACAGCTTGATGAGG | 60 |
| 30 | 923 | 1322 | CGCCTTACATCAATTCCTACAA | CGGTCATATTCGATCTGTTCC | 59 |
| 40 | 22 | 598 | ATTCTCGATCCGCATTTCAT | AGAGGCCGGAGAGAATAAGC | 60 |
Position of primers is relative to the gene set alignment