Table 1.
Primer design for the RT-PCR assay
| Gene | Primer | PCR product length (bp) | Sequence 5′ to 3′ | Tm (°C) |
|---|---|---|---|---|
| MSTN | F | 423 | GATTATCACGCTACCACG | 58.9 |
| R | ATTCAGCCCATCTTCTCC | |||
| ActRIIB | F | 163 | GCAGTCGTGGCAGAGTGAGCG | 51.7 |
| R | CTTGAGGTAATCCGTGAGGGAGC | |||
| Akt | F | 269 | TAGGCATCCCTTCCTTACAG | 55.8 |
| R | GCCCGAAGTCCGTTATCT | |||
| FoxO1 | F | 459 | TCAAGGATAAGGGCGACAG | 54.4 |
| R | GTGGATACACCAGGGAATG | |||
| mTOR | F | 368 | CTGGCTTCCAACCCTAAA | 53.5 |
| R | TCTCCAAATCCCACTCCC | |||
| GLUT4 | F | 545 | GTC ATC AAC GCC CCA CAG | 56.8 |
| R | TCA GGA CAG AAG GGC AAC AG | |||
| GAPDH | F | 292 | CGACTGTTAGAACTCCCTCA | 57.4 |
| R | CATTGGGGGTAGGAACAC |
F forward, R reverse