Table 2.

List of primers

No.	Gene code	Forward	Melting point of amplicon
		Reverse
1	T.bet	5′CCAACAATGTGACCCAGATGATT3′	85
		5′TATGCGTGTTGGAAGCGTTG3′
2	IL-12p40	5′GCCCAGAGCAAGATGTGTCA3′	82
		5′GGGCATCCGGATACCAATC3′
3	IFN-γ	5′TCAGCTCTGCATCGTTTTGG 3′	79
		5′GTTCCATTATCCGCTACATCTGAA3′
4	GATA3	5′CCCTACTACGGAAACTCGGTCA3′	88
		5′GTAGGGATCCATGAAGCAGAGG3′
5	IL-4	5′CAAGCAGCTGATCCGATTCC3′	81
		5′TTCTCTCTCATGATCGTCTTTAGCC3′
6	IL-5	5′CCTGTTCCTGTACATAAAAATCACCA3′	80
		5′TTGAATAGTCTTTCCACAGTACCCC3′
7	RORC	5′ACAGCACCGAGCCTCACG3′	85
		5′CAGACGACTTGTCCCCACAGA3′
8	IL-17	5′TTGATTGGAAGAAACAACGATGACT3′	81
		5′TGGATTTCGTGGGATTGTGAT3′
9	CCL22	5′TGCCGTGATTACGTCCGTTA3′	87
		5′CGGCACAGATCTCCTTATCCC3′
10	FOXP3	5′ACAGCACATTCCCAGAGTTCCT3′	82
		5′GATGAGCGTGGCGTAGGTG3′
11	CTLA4	5′TGGATCCTTGCAGCAGTTAGTTC3′	79
		5′CATTTTCACATAGACCCCTGTTGTA3′
12	IL-13	5′AGGTCTCAGCTGGGCAGTTTT3′	80
		5′TAATGATGCTTTCGAAGTTTCAGTTG3′
13	STAT3	5′CTCAAGAGTCAAGGAGACATGCA3′	85
		5′CTCACTCACGATGCTTCTCCG3′
14	FASL	5′CTCCGAGAGTCTACCAGCCAGAT3′	84
		5′CATGGACCTTGAGTTGGACTTG3′
15	CCR7	5′GTGGTGGCTCTCCTTGTCATTT3′	84
		5′ATGATAGGGAGGAACCAGGCTT3′
16	IL-6	5′CCTGAGAAAGGAGACATGTAACAAGAG3′	81
		5′GCAAGTCTCCTCATTGAATCCAG3′
17	VEGF	5′AGGAGGAGGGCAGAATCATCA3′	81
		5′CTCGATTGGATGGCAGTAGCT3′
18	IL-10	5′GTGATGCCCCAAGCTGAGA3′	86
		5′CACGGCCTTGCTCTTGTTTT3′
19	TGF-β	5′CCTGGACACCAACTATTGCTTCA3′	83
		5′TGCGGAAGTCAATGTACAGCTG3′
20	IDO1	5′CTCTGCCAAATCCACAGGAAA3′	79
		5′TCTCAACTCTTTCTCGAAGCTGG3′
21	SOCS1	5′CCCTGGTTGTTGTAGCAGCTTAA3′	80
		5′GGTTTGTGCAAAGATACTGGGTATATG3′
22	TNF-α	5′CCCAGGGACCTCTCTCTAATCA3′	84
		5′ATGGGCTACAGGCTTGTCACTC3′
23	ACTB	5′CAGCAGATGTGGATCAGCAAG3′	83
		5′GCATTTGCGGTGGACGAT3′
24	TFRC	5′ACCGGCACCATCAAGCT3′	80
		5′TGATCACGCCAGACTTTGC3′

Melting point of amplicon is calculated by melt curve analysis using SDS software v2.6 Applied Biosystems