Fig. 1.

P2Y12R, P2X7R, and TSPO expression in non-stimulated (non-sti), pro-inflammatory (pro-inf), and anti-inflammatory (anti-inf) polarized human adult microglia. Quantitative PCR analysis and western blot were performed on mRNA and cell lysate collected from non-stimulated, LPS-stimulated pro-inflammatory, and IL-4-stimulated anti-inflammatory human cultured microglia. Expression level of P2Y12R (a), P2X7R (b), and TSPO (c) was evaluated. P2X7R and TSPO were significantly upregulated in pro-inflammatory microglia, and P2Y12R was significantly upregulated in anti-inflammatory microglia (n = 10 donors; each sample is done in duplicate). Representative western blot (d) and quantification (e) of P2Y12R and P2X7R protein expression in non-stimulated, pro-inflammatory, and anti-inflammatory human microglia (n = 2 donors). Error bars represent SD. Each sample was done in duplicate. (one asterisk) p < 0.05, (two asterisks) p < 0.01, (three asterisks) p < 0.001