Fig. 8.

Autoradiography on acute rat EAE tissue and human MS tissue using P2Y12R and P2X7R radiotracers. Representative autoradiograms of carbon-11-labeled P2Y12R-ant on rat control and EAE tissues (a). Representative autoradiograms of tritium-labeled A-740003 (P2X7R specific antagonist) on rat control and EAE tissues (b). Quantification of binding of [¹¹C]P2Y12R-ant and [³H]A-740003 on rat control and EAE tissues (data presented is the summary of n = 3 animals per time point and three independent experiments) (c). Representative autoradiograms of [¹¹C]P2Y12R-ant on different human MS lesion types (d). Representative autoradiograms of tritium-labeled A-740003 (P2X7R specific antagonist) on different human MS lesion types (e). Quantification of binding of [¹¹C]P2Y12R and [³H]A-740003 on different human MS lesions (data presented is a summary of three independent experiments using three non-neurological controls, three active, two chronic active, and three chronic inactive MS lesions) (f)