Fig. 4.

Ectopic miR-302b expression modulates EMT markers and the Wnt/β-catenin pathway by regulating expression of EphA2. a miR-302b and EphA2 siRNA(si-EphA2) reduced morphological characteristics of EMT. b Western blot (left) andqRT-PCR(right) analyses showing decreased levels of N-cadherin, β-catenin,Snail, c-Myc, and Cyclin D1, as well as increased E-cadherin, in miR-302b-overexpressed AGS and SGC7901 cells, as compared with the miR-NC counterparts. c qRT-PCR(right) and western blot(left) analysis for N-cadherin, E-cadherin,β-catenin,Snail, c-Myc, and Cyclin D1 following transfection with EphA2 siRNA (si-EphA2) versus control(si-NC). d The mRNA and protein levels of N-cadherin,E-cadherin, β-catenin,Snail,c-Myc,CyclinD1 were determined by qRT-PCR and western blot analysis after co-transfection with miR-302b(or a control) and EphA2 (or a control) expression vectors in AGS and SGC7901 cells. β-actin served as an internal control