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. 2017 Dec 22;14:258. doi: 10.1186/s12974-017-1033-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Pretreatment with donepezil did not suppress the mRNA expression of both TNFR1 and TNFR2 in rodent microglial cells. a–c Bar graphs showing that pretreatment with 5 μM donepezil for 12 h did not affect the mRNA expression of both TNFR1 and TNFR2 in rat HAPI (a) and mouse primary (b, c) microglial cells we used. In addition, pretreatment with donepezil did not suppress the mRNA expression of both TNFR1 (b) and TNFR2 (c) stimulated by TNFα treatment (3 ng/mL) in mouse primary microglial cells. NS > 0.1 vs control. Note that the unit of TNFR1 is 10 to the minus 3rd power in a