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. 2017 Dec 22;36:190. doi: 10.1186/s13046-017-0661-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunofluorescent images of HCT116 cells treated with or without 5-Fu. a. Hoechst 33,342 staining (blue) indicates nucleus and Cyto-ID Green staining (green) autophagy status. b. Western blot analysis of beclin-1, p62 and LC3II/LC3I in HCT116 cells after exposing to varied concentrations of 5-Fu for 24 h. *, p < 0.05, and **, p < 0.01, compared to the vehicle (0 μM 5-Fu) cell group