Fig. 5.

Repetitive ODE treatments induce memory B cell subpopulations. WT and BCR KO mice were repetitively exposed to saline or ODE daily for 3 weeks whereupon BALF and dissociated tissue cells were processed and analyzed by flow cytometry. Lymphocytes identified by CD45⁺ leukocytes excluding debris and characteristic FSC and SSC properties of lymphocytes followed by staining for CD19, CD73, and CD273. A representative contour plot of a BALF cells and c lung tissue cells demonstrating gating strategy for memory B cell populations from WT saline and ODE treated animals is shown. Numbers of memory B cells were calculated by multiplying the percentage of cells in respective gate (% of CD45+ cells, as analyzed by FACS) multiplied by respective total cells (see Fig. 3) for each mouse. Bar graphs depict means with standard error bars of b BALF and d lung tissue cells of 6 mice/group. There were no B cells in the BCR KO mice. Statistical difference (*p < 0.05; **p < 0.01) versus saline