Table 5.
APOE and TREM2 Models
| Model | Allele | Targeting approach | Availability | Reference |
|---|---|---|---|---|
| ApoE Lines | ||||
| GFAP-ApoE3 Line 37, GFAP-ApoE4, Line 1 | huAPOE3 or E4 cDNA | Transgenic, huGFAP promoter | JAX 004633, 004631 | [196] |
| ApoE2, E3, E4 KI | huAPOE2, E3, or E4 | Targeted insertion of APOE cDNAs | NA | [197] |
| ApoE KO | Apoe deletion (exon 3 replacement) | Targeted neo insertion replacing part of Apoe exon 3 | JAX 002052; 014556 | [193, 221] |
| APOE*3, E*4 KI | huAPOE3 or E4 | Targeted replacement of Apoe exons 2–4 | JAX 027894 (E4) JAX 029018 (E3) | NA |
| APOE2, E3, E4 Targeted replacement | huAPOE2, E3, or E4 | Targeted replacement of Apoe exons 2–4 | Taconic | [199–201] |
| TREM2 Lines | ||||
| TREM2 KO | Trem2 deletion (exon 2–3 deletion) | Targeted lacZ/neo replacement of TREM2 exons 2–4 | UCD/KOMP VG10093 | [206] |
| TREM2−/− | Trem2 deletion (exon 2–3 deletion) | Targeted deletion TREM2 exons 3–4 | Marco Colonna | [205] |
| TREM2 KO | Trem2 deletion (Q17X) | CRISPR/Cas9 targeted deletion |
JAX 027197 | NA |
| TREM2 R47H KI | endogenous mouse Trem2 (R47H) | CRISPR/Cas9 targeted mutation |
JAX 027918 | NA |
| TREM2 Y38C KI | endogenous mouse Trem2 (Y38C) | CRISPR/Cas9 targeted mutation |
JAX 029725 | NA |
| TREM2 p.T66 M | endogenous mouse Trem2 (T66 M) | CRISPR/Cas9 targeted mutation |
Christian Haass | [208] |
| TREM2 flox | Trem2 tm1c(EUCOMM)Wtsi loxP-flanked mouse Trem2 exons 2–3 | Targeted insertion | JAX 029853 | NA |