Table 1.
Cellulase A production and yield in different fermentation media over different phases (exponential growth phase versus early stationary phase and late stationary phase)
| Medium | End of Exponential phase | Early stationary phase | Late stationary phase | ||||||
|---|---|---|---|---|---|---|---|---|---|
| CelA concentration (mg/L) |
Yield of CelA on biomass (mg/gDCW) |
Fermentation time (h) | CelA concentration (mg/L) |
Yield of CelA per unit of biomass (mg/gDCW) |
Fermentation time (h) |
CelA concentration (mg/L) |
Yield of CelA per unit of biomass (mg/gDCW) |
Fermentation time (h) |
|
| MM | 6.8 | 2.5 | 33 | 12.9 | 4.5 | 48 | – | – | – |
| MMC5 | 12.3 | 2.7 | 28 | 22.0 | 5.5 | 44 | – | – | – |
| MMC15 | 1.5 | 0.4 | 23.3 | 15.7 | 2.8 | 35 | – | – | – |
| TSB | 1.1 | 0.4 | 17 | 5.4 | 2.1 | 35 | – | – | – |
| Bennet | 4.9 | 1.5 | 65 | 4.9 | 2.2 | 67.2 | – | – | – |
| NB | 19.2* | 22.4** | 28 | 33.5* | 37.7** | 48 | 72.1 | 119.6 | 168 |
| NB2X | 27.5* | 13.9** | 23 | 60.8* | 25.2** | 42 | – | – | – |
The italics numbers indicate the difference in CelA amounts produced in NB and NB2x media
MM, minimal medium; C5 and C15, casamino acids (5 or 15 g/L); TSB, tryptic soy broth; NB, nutrient broth; NB2x, nutrient broth double strength; DCW, dry cell weight. n = 3
* The changes in CelA amount in (mg/L). The amount significantly increases with doubling the amount of media
** The changes in CelA amount in (mg/ g DCW). The amount of CelA significantly decreases with doubling the amount of the media