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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Nanomedicine. 2017 Sep 7;14(1):63–71. doi: 10.1016/j.nano.2017.08.018

Figure 5.

Figure 5

Confocal imaging of live MRMECs treated with AS-VCAM-1 hAuNP and NS hAuNP. Cells were cultured on microscope slides and treated with TNF-α or vehicle plus AS-VCAM-1 hAuNP or NS hAuNP in complete growth medium. After a 6-hour incubation, these media were aspirated and fresh medium was added to each culture. The cells were imaged using confocal microscopy. (A) Strong fluorescence emission was only detected in TNF-α-activated MRMEC treated with AS-VCAM-1 hAuNP. (B, C and D) Only minimal fluorescence was detected in the other cultures. (E) Fluorescence intensity is expressed in relative fluorescence units (RFU) per cell over different treatment groups as measured using ImageJ software.