Table 3.

Comparison of the replot and numerical methods.

	System	Method		KI (μM)	kinact (min−1)	kinact/KI (μM−1, min−1)	R2
−DEX	RLM	Replot	All data points	1.13 ± 0.45	0.012 ± 0.001	0.011 ± 0.004	0.984
			Linear	1.45 ± 0.46	0.027 ± 0.003	0.019 ± 0.006	0.9996
		Numerical	Direct	1.35 ± 0.57	ND	ND	0.962
			Seq	2.37 ± 1.06	ND	ND	0.967
	SRH	Replot	All data points	1.15 ± 0.05	0.016 ± 0.0002	0.014 ± 0.0006	0.9998
			Linear	1.35 ± 0.12	0.058 ± 0.002	0.043 ± 0.004	0.999
		Numerical	Direct	10	ND	ND	0.850
			Seq	10	ND	ND	0.943
+ Dex	RLM	Replot	All data points	2.79 ± 0.47	0.050 ± 0.004	0.018 ± 0.003	0.998
			Linear	5.99 ± 1.72	0.11 ± 0.017	0.018 ± 0.006	0.996
		Numerical	Direct	4.41 ± 0.87	ND	ND	0.973
			Seq	5.4 ± 1.1	0.012 ± 0.010	0.002 ± 0.002	0.974
	SCRH	Replot	All data points	0.19 ± 0.14	0.011 ± 0.001	0.058 ± 0.043	0.985
			Linear	1.73 ± 0.39	0.041 ± 0.003	0.024 ± 0.006	0.995
		Numerical	Direct	10	ND	ND	0.795
			Seq	10	ND	ND	0.907
	SRH	Replot	All data points	6.35 ± 1.9	0.040 ± 0.007	0.0063 ±	0.994
			Linear	12.7 ± 4.6	0.16 ± 0.04	0.012 ± 0.005	0.995
		Numerical	Direct	10	0.007 ± 0.006	0.0007 ± 0.0006	0.918
			Seq	10	0.009 ± 0.005	0.0009 ± 0.0005	0.919