Figure 4.

Memory generation depends on toll-like receptor 7 signaling in B cells. (A) Splenic B cells from C57BL/6 or Tlr7^−/− mice were transferred into muMT recipients. One day later, mice were immunized, and spleens were analyzed at 14 days. (B) NIP-binding B cells were gated for mutated memory cells (B220⁺NIP⁺CD80⁺CD35^lo). (C) Memory B cells from C57BL/6 or Tlr7^−/− donors. Each symbol depicts one muMT mouse from four independent experiments with two mice each. Bar indicates mean; p-value determined by one-tailed Student’s t-test.