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. 2017 Dec 19;8:930. doi: 10.3389/fphar.2017.00930

FIGURE 1.

FIGURE 1

Determination of LD50 of diethylaminoethyl dextran (DEAE-Dextran) by MTT cell proliferation assay; (A) % cell proliferation inhibition in MCF-7 cell line and (B) % cell proliferation inhibition in MDA-MB-231 cell line. Trypan blue cell viability assay; (C) The % cell death at 6 h post-treatment (D) % cell death 12 h post-treatment (E) % cell death 24 h post-treatment. Reactive oxygen species (ROS) measurement post DEAE-Dextran treatment; (F) ROS in HEK293 cells without peroxide pre-incubation, (G) ROS in MDA-MB-231 cells without peroxide pre-incubation, (H) ROS measurement in HEK293 cells with hydrogen peroxide pre-incubation and (I) ROS measurement in MDA-MB-231 cells with hydrogen peroxide pre-incubation. # Significantly different from control (P < 0.05), Values expressed as Mean ± SEM. H2O2 Control – hydrogen peroxide (100 μM) treated cells, P1 – 1 μM paclitaxel, P2 – 5 μM paclitaxel, D1 – 1 μM DEAE-Dextran, D2 – 5 μM DEAE-Dextran.