Figure 3.

Skeletal muscle weakness in mice with 4T1 osteolytic cancer in bone (OCIB). (A) In vivo forelimb grip strength (n = 10) and (B) excised whole muscle contractility of the extensor digitorum longus (EDL) muscle (n = 10) were lower in mice with 4T1 OCIB. (C) Skeletal muscle fatigue was higher in mice with 4T1 cells in bone (percent of maximum force) (n = 10). (D) Half-relaxation time (1/2 RT) was higher in EDL from mice with 4T1 OCIB compared to non-tumor control mice. Data are mean ± SEM, (A,B,C) two-way analysis of variance with Bonferroni post-test, (D) t-test. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.