FIGURE 8.

Mature form of DY1 is further processed during the development of leaves. (A) A diagram showing the pre-protein structure of the DY1 protein labeled with dual fluorescent proteins. (B) Western blot analysis of the quantities of both ends of DY1 labeled with dual fluorescent proteins. Total proteins were extracted from 1- and 2-week-old transgenic Arabidopsis seedlings. (C) Transient expression of the eYFP-DY1-mCherry fusion protein in tobacco leaves demonstrating a possible cleavage of the C-terminus of DY1 during leaf development. Bars = 10 μm. (D) Fluorescent observation of 2-week-old Arabidopsis seedlings showing the lack of mCherry signals. Bars = 5 μm. In (C) and (D), the Profile panels (green line, eYFP signal; orange line, mCherry signal) demonstrate the signal intensity profiles along the vertical lines indicated in the Merge panels.