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. 2017 Dec 19;8:2553. doi: 10.3389/fmicb.2017.02553

FIGURE 3.

FIGURE 3

(A) Gelatin zymography assay of wild-type strain RA-YM, mutant strain ΔsprT, and complemented strain CΔsprT. Regions of clearing reflect protease activity. Black arrows indicate distinct protease activity present in RA-YM and ΔsprT. These bands were excised for identification by MS. (B) SDS-PAGE analysis of recombinant RAYM_01812 protein incubated at 37°C for different times. M: protein marker; lane 1: purified GST-RAYM_01812 fusion protein; lanes 2–7: purified GST-RAYM_01812 fusion protein incubated at 37°C for 0.5 h (lane 2), 1 h (lane 3), 4 h (lane 4), 8 h (lane 5), 24 h (lane 6), and 48 h (lane 7). (C) Ability of purified RAYM_01812 protein to degrade gelatin after incubation at 37°C for different times. M: protein marker; lane 1: purified GST-RAYM_01812 fusion protein; lanes 2–7: gelatin zymography analysis of GST-01812 fusion protein incubated at 37°C for 0.5 h (lane 2), 1 h (lane 3), 4 h (lane 4), 8 h (lane 5), 24 h (lane 6), and 48 h (lane 7).