Table 1.
Strains, plasmids, and primers used in this study.
| Strains, plasmids, and primers | Descriptions | Source |
|---|---|---|
| Strains | ||
| RA-YM | Riemerella anatipestifer wild-type strain, serotype 1 | Preserved in the laboratory |
| ΔsprT | sprT gene deletion mutant strain, SpcR | This study |
| CΔsprT | Complemented RA-YM aaasprT strain, SpcR, AmpR | This study |
| E. coli DH5α | Competent cell | Transgen |
| E. coli X7213 | Competent cell | This study |
| Plasmids | ||
| pMD18-T | TA cloning vector | Takara |
| pRE112 | Suicide vector | Preserved in the laboratory |
| pRE112-LSR | Suicide vector | This study |
| pRES-JX-bla | Shuttle vector | Preserved in the laboratory |
| RA-JX | Wild type plasmid of RA | Preserved in the laboratory |
| pRES-JX-bla-sprT | Shuttle vector | This study |
| pGEX-6P-1 | Expression vector | Preserved in the laboratory |
| pGEX-6P-1_01812 | Expression vector | This study |
| Primers (5′–3′) | ||
| Spc-F1 | CAGTGGAACGAAAACTCACGTT | This study |
| Spc-R1 | CAGTAGTTTTAAAAGTAAGCACCTG | This study |
| SprT-F1 | ACGGGTACCAGGTACTAAAGCCGAAAT | This study |
| SprT-R1 | ACGTGAGTTTTCGTTCCACTGCATGG CAGAAATACTAATG | This study |
| SprT-F2 | TGCTTACTTTTAAAACTACTATACAC GCTGATAGATGG | This study |
| SprT-R2 | CGAGCTCCAAACCTCGGTAAACAAA | This study |
| SprT-IS | ATGGACAGAATGGAAGGC | This study |
| SprT-IR | TTTTGGAGTGAATGGAGC | This study |
| Promoter-sprT1 | GGGTACCAAGTATTTTGGATAAATTAGACT | This study |
| Promoter-sprT2 | TATCTTTTTCATTAATCTTAAAAATTTACAGCCAC | This study |
| SprT-CF | TTTTTAAGATTAATGAAAAAGATATTTTTTTTAAC | This study |
| SprT-CR | GGCATGCTTATTCAAATTTTAGCACAAACA | This study |
| P01812-1 | CGGGATCCATGGCTCAAAACCAAAACACATC | This study |
| P01812-2 | CCCTCGAGCTTCTTGATAAATTTCTTAGTAA | This study |
RResistance.