Figure 4.

Selective permeability of Bruch’s membrane (BrM) to complement analphylatoxins and antibodies (A,B) Purified C5a diffused across enriched BrM over 24 h, while (C) no purified C3a was detected in the diffusate chamber after 24 h. (D) Whole IgG did not cross enriched BrM samples, whereas (E) the Fab antibody fragment ranibizumab did cross enriched BrM, but did not reach equilibrium after 24 h, where only an average of ~12% of the Fab antibody was detected in the diffusate chamber (F). Data shown are representative of five separate experiments; error bars in (B) and (F) represent ± SD.