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. 2017 Dec 19;11:358. doi: 10.3389/fncel.2017.00358

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Copyright © 2017 Liu, Liu, Chao, Liu, Liu, Zheng, Chen, Gong, Teng, Li, Wang and Xue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of overexpression or silencing of miR-330-3p on the expression levels of PKCα and p-PKCα in EMAP-II treated GECs. (A) The relative mRNA expression level of PKCα was detected by real-time quantitative PCR. U6 was used as an inner control. (B) Western blot analysis of the PKCα and p-PKCα levels in GECs. GAPDH was used as an inner control. *P < 0.05, **P < 0.01 vs. control group; ^#P < 0.05, ^##P < 0.01 vs. EMAP-II group. (C) The expression and distribution of PKCα in GECs was analyzed by immunofluorescence assay. Scale bar represents 20 μm. Values are means ± SD (n = 5, each).