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. 2017 Dec 19;11:358. doi: 10.3389/fncel.2017.00358

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Copyright © 2017 Liu, Liu, Chao, Liu, Liu, Zheng, Chen, Gong, Teng, Li, Wang and Xue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-330-3p combined with PKCα activator or PKCα inhibitor affect BTB permeability and the expression levels of TJ related proteins. (A,B) TEER and HRP assays were used to measure the permeability of BTB. (C) The relative mRNA expression levels of ZO-1, occludin, and claudin-5 were detected by real-time quantitative PCR. U6 was used as an inner control. (D) The protein expression levels of ZO-1, occludin and claudin-5 were determined by Western blot. GAPDH was used as an inner control. Values are means ± SD (n = 5, each). *P < 0.05 vs. control group; ^#P < 0.05 vs. pre-miR-330-3p group; ^$P < 0.05 vs. anti-miR-330-3p group.