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. 2017 Dec 19;11:358. doi: 10.3389/fncel.2017.00358

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Copyright © 2017 Liu, Liu, Chao, Liu, Liu, Zheng, Chen, Gong, Teng, Li, Wang and Xue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EMAP-II combined with anti-miR-330-3p and PKCα activator increases BTB permeability and the expression levels of TJ related proteins in GECs. (A,B) TEER and HRP assays were used to measure the permeability of BTB. (C) The protein expression levels of ZO-1, occludin and claudin-5 were determined by Western blot. GAPDH was used as an inner control. Values are means ± SD (n = 5, each). *P < 0.05, **P < 0.01 vs. control group; ^#P < 0.05 vs. EMAP-II group. (D) The expression and distribution of ZO-1, occludin and claudin-5 in GECs was analyzed by immunofluorescence assay. Scale bar represents 20 μm.