Table 2.
Primers and probe sequences used in ChIP analysis, including gene symbol and Ensembl gene ID for the target genes.
| Gene symbol | Ensembl gene ID | Forward primer sequence | Reverse primer sequence | Probe sequence |
|---|---|---|---|---|
| c-Fos SRE | ENSRNOG00000008015 | TCAATCCCTCCCTCCTTTACAC | GGTTCCCCCCAGGACAAC | AGGTTTCCACGGCCGGTCCC |
| c-Fos CRE | ENSRNOG00000008015 | TTCCCCCCTCCAGTTTCTCT | TCAGCTGGCCGCTTTATAGAAG | TTCCGCTCATGACGTAGTAAGCCATTCAA |
| Egr-1 SRE only | ENSRNOG00000019422 | GACCCGGAAACACCATATAAGG | AAGGCGCTGCCCAAATAAG | AAGGATCCCCCGCCGGAACAG |
| Egr-1 CRE/SRE | ENSRNOG00000019422 | GCTCTTGGATGGGAGGTCTTC | TCCGCCGTGACGTACATG | TCCTCCCGGTCGGTCCT |
| BDNF CRE∗ | ENSRNOG00000047466 | TCATGACACCAGACAAATCCTGT | TCTCCCTAGAGGCAGTGTGACA | TTCCCTATCTGAAAAACACCGTGGTCCG |
∗CRE site (TGACGGC) predicted by Genomatix software located near the transcriptional start site of exon IX in BDNF.