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. 2017 Dec 19;11:409. doi: 10.3389/fncel.2017.00409

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Copyright © 2017 Perny, Ting, Kleinlogel, Senn and Roccio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neuronal differentiation transits through otic developmental steps. Gene expression analysis of the spiral ganglion development-related genes Sox2, Dlx5, Eya1, Pax8, Pax2, Neurogenin 1, Neurogenin 2, NeuroD, GATA3, Prox1, Islet1, p75, Prikle1, Mafb, and Peripherin at the indicated days in culture in differentiation conditions. Values are fold induction versus undifferentiated mESCs (d0, set as 1). For each time point, the average fold induction obtained in three to five independent experiments is shown. d0 (n = 5); d5 (n = 3); d8 (n = 5); d12 (n = 4); and d15 (n = 3). Error bars are SEM. Black (ANOVA with multiple comparison correction, Tukey’s test); Gray bars (ANOVA w/o multiple comparison correction: Fisher’s LSD test). (^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005).