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. 2017 Dec 19;8:2138. doi: 10.3389/fpls.2017.02138

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Copyright © 2017 Moriceau, Jomat, Bressanelli, Alcaide-Loridan and Jupin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of the structure and amphipathic property of CTD α-helices on membrane association of 98K in vivo. Arabidopsis protoplasts were transfected with the expression plasmids pΩ-98K, pΩ-98K-αA-αB(LL/PP) or pΩ-98K-αA-αB(ILLL/AAAA) as indicated. The cells were harvested 24 hpt and were lyzed to generate a total protein fraction (T). Soluble (S) and insoluble pellet (P) fractions were then separated by centrifugation, and samples of each fraction corresponding to equal amount of fresh tissue were subjected to 8% SDS-PAGE and immunoblot analysis using anti-98K antiserum. Molecular mass markers (Biolabs) are indicated on the left, whereas positions of the viral proteins 98K and 85K are indicated on the right.