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. 2017 Dec 19;8:2138. doi: 10.3389/fpls.2017.02138

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Copyright © 2017 Moriceau, Jomat, Bressanelli, Alcaide-Loridan and Jupin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of the structure and amphipathic property of CTD α-helices on viral infectivity. Arabidopsis protoplasts were transfected with in vitro transcripts as indicated, and cells were harvested 48 hpt. (A) The ability of the transcripts to replicate was assessed by extracting total RNAs and quantifying viral genomic RNA by RTqPCR. The relative accumulation of viral mutant RNAs as compared to the E17-stop69K control is represented as the mean ± SD. Mean and SD values, as well as the number of samples (n) analyzed in two independent experiments are indicated below panel (A). (B) The ability of the transcripts to replicate was assessed by subjecting total protein samples to 15% SDS-PAGE and immunoblot analysis using anti-CP antiserum. Molecular mass markers (Biolabs) are indicated on the left whereas position of CP is indicated on the right. Ponceau staining of the membrane (staining) indicates protein loading.