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. 2017 Dec 19;8:2138. doi: 10.3389/fpls.2017.02138

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Copyright © 2017 Moriceau, Jomat, Bressanelli, Alcaide-Loridan and Jupin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of the structure and amphipathic property of CTD α-helices on the ability of 98K to interact with 66K as determined by bimolecular fluorescence complementation. (A) Arabidopsis protoplasts were cotransfected with the expression plasmids as indicated, together with pΩ-RbCS-NiRFP. Cells were collected 40 hpt and the percentage of cells displaying a fluorescent YFP signal was determined by flow-cytometry. Values were normalized to the percentage of transfected cells and are represented as the mean ± SD of three independent experiments. (B) Single protoplasts were observed by spinning-disk confocal laser microscopy (SPCLM) 48 hpt and YFP localization was observed (green). To visualize the localization of chloroplasts, NiRFP fluorescence and chlorophyll autofluorescence were acquired (magenta) and superimposed onto the YFP fluorescence. Scale bars, 10 μm.