Figure 2.

Effect of acute heat stress on the expression of HSP70 and orexin system in QM7 cell lines. Protein levels of HSP70, ORX, and ORXR1/2 were determined by Western blot (A) and their relative expression was presented as normalized ratio of target protein/actin (C). Relative abundance of ORX and ORXR1/2 mRNA (B) was measured by real-time RT-PCR. Protein localization and expression was also assessed by immunofluorescence (D). Data are presented as mean ± SEM (representative of at least three replicates). ^* and different letters indicate significant difference at P < 0.05.