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. 2017 Dec 19;8:1082. doi: 10.3389/fphys.2017.01082

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Copyright © 2017 Jana, Meng, Hirsch, Friedman and Alayash.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Oxidized hemoglobins cause bioenergetic reprogramming in pulmonary endothelial cells. HPAEC were exposed to ferryl forms of HbA or HbS or HbE at equimolar concentration (100 μM) for 12 h. Mitochondrial oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) were measured in real time using XF24 extracellular flux analyzer. (A) Average maximal OCR values expressed as % of control in HPAEC obtained after uncoupling of mitochondria following FCCP treatment (N = 3). (B) Average glycolytic reserve capacity expressed as (%) of control in HPAEC (N = 4) (C) representative ECAR plots of ferryl Hb treated HPAEC from an individual set of experiment repeated three times. Statistical significance between means was calculated by paired Student's t-test. ^*P < 0.05 vs. untreated control.