Figure 2.

NbKPILP expression was observed in the roots but not in intact leaves. (A) View of a typical 6-week-old N. benthamiana plant used to analyse the content of RNA and protein. (B) Western blot analysis with NbKPILP-specific antibodies of total protein extracts from leaves and roots. The lower panel shows the protein loading control stained with Amido Black. rNbKPILP, recombinant NbKPILP with 6xHis-tag produced in E. coli. (C) Relative amounts of NbKPILP mRNA in roots and leaves quantified by qRT-PCR.