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. 2017 Dec 19;8:2137. doi: 10.3389/fpls.2017.02137

Figure 5.

Figure 5

Overproduction of GFP as a foreign protein in the leaves stimulates the accumulation of NbKPILP mRNA. (A) Schematic representation of the crTMV-based vector crTMV:GFP and its intron-containing variant crTMV(i):GFP. Act2, Arabidopsis thaliana act2 gene promotor; genes encoding replicase, movement protein (MP) and GFP, the 3′-untranslated region are shown; nos T stands for terminator of transcription. Introns are denoted by light rectangles. (B) UV visualization of GFP in leaves infiltrated with crTMV:GFP (upper part of the leaf) and crTMV(i):GFP (lower part of the leaf) on the 4th day after infiltration. (C) Relative amounts of GFP mRNA in N. benthamiana leaves agroinjected with crTMV-based vectors quantified by qRT-PCR. GFP mRNA accumulation mediated by crTMV:GFP vector was set as 1. The statistical significance of the difference between GFP mRNA levels mediated by two crTMV-based vectors is shown. ***P < 0.001 (Student's t-test). (D) Relative amounts of NbKPILP mRNA in N. benthamiana leaves agroinjected with crTMV-based vectors quantified by qRT-PCR. NbKPILP mRNA level of the intact leaf was set as 1. Control, leaf agroinfiltrated with empty vector pBin19. The statistical significance of the difference between NbKPILP mRNA levels mediated by two crTMV-based vectors is shown. ***P < 0.001 (Student's t-test).