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. 2017 Dec 19;8:2137. doi: 10.3389/fpls.2017.02137

Figure 7.

Figure 7

NbKPILP mRNA contains an ANRF translated into short 53-aa polypeptide (53aa-ANRF) in N. benthamiana leaves. (A) The sequence of the NbKPILP cDNA with the designated potential start codons (bold). Polypurine block sequence is highlighted in bolded red; 53aa-ANRF is italicized and underlined. (B,C) Schematic representation of the 35S-NbKPILP(53aa-GFP) (B) and 35S-(SS-)NbKPILP(53aa-GFP) (C) vectors comprising the following elements: CaMV 35S promoter, NbKPILP with or without 24-aa signal sequence (SS-) and 53aa-ANRF fused with GFP. (D,E) Western blot analysis using anti-GFP antibodies of N. benthamiana leaf proteins at 3 days after agroinjection with the 35S-NbKPILP(53aa-GFP) (D) or 35S-(SS-)NbKPILP(53aa-GFP) (E) vectors. Cell homogenate was fractionated into P1, P17, S17, and CW fractions. G, the total soluble protein from leaves 3 days after agroinjection with 35S-GFP vector. The lower panels show the protein loading control stained with Amido Black. All agroinfiltrations were performed in the presence of a vector encoding silencing suppressor P19 from Tomato bushy stunt virus.