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. 2017 Dec 20;11:413. doi: 10.3389/fncel.2017.00413

Figure 7.

Figure 7

The effect of U0126 on the hippocampal properties. (A) Representative western blot for TRPC6, Kv4.3, ERK1/2 and pERK1/2. U0126 effectively decreases pERK1/2 levels, but not others. (B) Quantification of protein expression and phosphorylation based on the western blots. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM (*p < 0.05 vs. vehicle; n = 7, respectively). (C) Representative photos demonstrating Kv4.3 expression in the hilar interneurons. As compared to control vehicle, U0126 markedly reduces the dendritic Kv4.3 localization. Bar = 12.5 μm. (D) Representative western data demonstrating the effect of U0126 on Kv4.3 subcellular locations. U0126 reduces membrane translocation of Kv4.3, but increases cytosolic Kv4.3 intensity. (E) Quantification of the effect of U0126 on membrane Kv4.3 translocation. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM (*p < 0.05 vs. vehicle; n = 7, respectively). (F) Quantification of the effect of U0126 on cytosolic Kv4.3 intensity. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM (*p < 0.05 vs. vehicle; n = 7, respectively). (G) Representative traces of paired-pulse responses in the dentate gyrus of vehicle- and U0126-treated animals at 30 ms interstimulus interval at stimulus intensity 2× threshold. (H) Normalized excitability ratio in the dentate gyrus of vehicle- and U0126-treated animals. *p < 0.05 vs. vehicle-infused animals (mean ± SEM; n = 7, respectively). (I) Normalized population spike amplitude ratio in the dentate gyrus of vehicle- and U0126-treated animals (mean ± SEM; n = 7, respectively). (J) Representative traces of paired-pulse responses in the CA1 region of vehicle- and U0126-treated animals at 30 ms interstimulus interval at stimulus intensity 2× threshold. (K) Normalized fEPSP amplitude ratio in the CA1 region of vehicle- and U0126-treated animals (mean ± SEM; n = 7, respectively).