Table 1.

IMGT/HighV-QUEST analysis of the scFv Pacific Biosciences (PacBio) reads.

PCR sample no.	Number of reads with 99.9% predicted accuracy	Mean number of passes	Number of movies	SMRT cell no.	Number of PacBio CCS2 analyzed reads	Number of scFv candidates in analyzed reads	% of scFv candidates in analyzed reads, i.e., coverage	Number of filtered-in reads	% of scFv in filtered-in reads, i.e., coverage	% of scFv in analyzed reads, i.e., coverage
s1	91,828	24	3	1	29,224	25,419	86.98	22,906	90.11	78.38
				2	32,240	28,120	87.22	25,228	89.72	78.25
				3	30,364	26,496	87.26	23,799	89.82	78.38
s2	129,640	23	4	4	34,082	29,729	87.23	26,657	89.67	78.21
				5	33,510	29,213	87.18	26,407	90.39	78.80
				6	31,980	27,874	87.16	25,032	89.80	78.27
				7	30,068	26,289	87.43	23,695	90.13	78.80
s3	115,446	24	4	8	34,890	30,183	86.51	26,990	89.42	77.36
				9	29,373	25,314	86.18	22,468	88.76	76.49
				10	26,465	22,776	86.06	20,044	88.00	75.74
				11	24,718	21,358	86.41	18,741	87.75	75.82
s4	113,644	24	4	12	25,128	21,881	87.08	19,120	87.38	76.09
				13	23,693	20,515	86.59	17,756	86.55	74.94
				14	32,293	28,093	86.99	24,762	88.14	76.68
				15	32,530	28,395	87.29	25,054	88.23	77.02
Total	450,558				450,558	391,655	86.93	348,659	89.02	77.38

Each CCS read counts as “1× coverage” over the scFv molecules of interest. The coverage is given in percentage of the scFv of interest (analyzed reads, filtered-in reads and analyzed reads).