Table 3.

Mutations observed at the 5′ and 3′ end of the seven Pacific Biosciences (PacBio) reads with 100% identity on 939 bp (positions 3–941).

PacBio read no. (assigned in the list 1–85)	PCR sample no.	SMRT cell no.	Mutation descriptiona	Mutation localization	GenBank/ENA/DDBJ accession number
13	s1	2	One 1 nt-deletion (g2 > del)	5′ end of the 5′ primer	MG272209
11	s1	2	Two 1 nt-deletion (t975 > del, a977 > del)	3′ end of the 3′ primer	MG272210
15	s1	3	One 1 nt-substitution (c956 > t)	Vicinity of the 3′ primer	MG272211
12	s1	2	One 1 nt-deletion (a942 > del)b	Vicinity of the 3′ primerb	MG272212
35	s2	5
71	s4	12
64	s3	10	Two 1 nt-deletion (a942 > del),b (t975 > del)	Vicinity of the 3′ primer,b 3′ end of the 3′ primer	MG272213

Positions of the primers are 1–23 and 958–977.

^aMutations are described according to the IMGT Scientific chart rules (http://www.imgt.org/IMGTScientificChart/Nomenclature/IMGTmutation.html) (40).

^bThe 1 nt-deletion (a942 > del) found in reads from the four samples most probably originates from the library.