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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Nat Protoc. 2017 Aug 17;12(9):1871–1889. doi: 10.1038/nprot.2017.071

Figure 5. Monovalent DNA conjugation of MPNs.

Figure 5

(a) Schematic illustration of oligonucleotide conjugation and purification of monovalent MPNs. Monovalent MPNs can be isolated via charge-based valency discrimination in an anion exchange HPLC column. To enhance colloidal stability and increase separation yield, thiolated PEG is used as an additional coating. (b) Oligo-length dependent HPLC elution profiles of MPNs. 30, 60, 80 and 100 bases DNA oligos are conjugated to 40 nm MPNs.