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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Nat Protoc. 2017 Aug 17;12(9):1871–1889. doi: 10.1038/nprot.2017.071
Time (hh:mm) Temperature Process
00:00 – 00:25 RT – 200 °C Gradual heating
00:25 – 01:25 200 °C Constant temperature
01:25 – 02:25 200 °C – 330 °C Gradual heating
02:25 – 330 °C – RT Gradual cooling