Table 1.

Forward and reverse primers used for real-time PCR.

Gene	Forward primer	Reverse primer	Ref
GAPDH	5′ AGAAGGCTGGGGCTGATTTG 3′	5′ AGGGCCCATCCACAGTCTTC 3′	[33]
COL I	5′ GGCTCCTGCTCCTCTTAGCG 3′	5′ CATGGTACCTGAGGCCGTTC 3′	[25]
COL III	5′ CAGCGGTTCTCCAGGCAAGG 3′	5′ CTCCAGTGATCCCAGCAATCC 3′	[25]
DCN	5′ CGCCTCATCTGAGGGAGCTT 3′	5′ TACTGGACCGGGTTGCTGAA 3′	[25]
ACAN	5′ CACTGTTACCGCCACTTCCC 3′	5′ ACCAGCGGAAGTCCCCTTCG 3′	[34]
TCN	5′ GGTGGATGGATTGTGTTCCTGAGA 3′	5′ CTGTGTCCTTGTCAAAGGTGGAGA 3′	[25]
SCX	5′ ACACCCAGCCCAAACAGA 3′	5′ GCGGTCCTTGCTCAACTTTC 3′	[25]
TNMD	5′ CCATGCTGGATGAGAGAGGT 3′	5′ CTCGTCCTCCTTGGTAGCAG 3′	[34]
RUNX2	5′ CAACCACAGAACCACAAGTGC 3′	5′ TGTTTGATGCCATAGTCCCTCC 3′	[25]
ALP	5′ GATCTTCTTTCTCCTTTGCCTGG 3′	5′ TGTTTGCAGTGGTGGTTCTGGCA 3′	[26]
COL II	5′ GGCAATAGCAGGTTCACGTACA 3′	5′ CGATAACAGTCTTGCCCCACTT 3′	[35]
Sox 9	5′ CACACAGCTCACTCGACCTTG 3′	5′ TTCGGTTATTTTTAGGATCATCTCG 3′	[26]
MyoD	5′ GCAGGTGTAACCGTAACC 3′	5′ ACGTACAAATTCCCTGTAGC 3′	[36]
MYOG	5′ GCCACAGATGCCACTACTTC 3′	5′ CAACTTCAGCACAGGAGACC 3′	[36]