Table 1.
Lipid concentrations in DRM are modified differentially between WT SR-B1 and SR-B1-Q445A after PPM supply
| Lipids | WT SR-B1 (mM, mean ± SEM) | SR-B1-Q445A (mM, mean ± SEM) | WT vs WT PPM | Q445A vs Q445A PPM | |||||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| subclass | species | - | PPM | - | PPM | variation | p value | variation | p value |
| SM | d18:0/16:0 | 3.44 ± 0.07 | 4.04 ± 0.17 | 10.21 ± 0.04 | 10.19 ± 0.42 | +17% | 0.03 | - | 0,96 |
| d18:1/18:0 | 0.38 ± 0.02 | 0.36 ± 0.01 | 0.58 ± 0.02 | 0.50 ± 0.01 | - | 0.4 | −14% | 0.02 | |
| d18:1/24:1 | 2.68 ± 0.35 | 2.42 ± 0.17 | 4.23 ± 0.15 | 3.58 ± 0.13 | - | 0.54 | −15% | 0.03 | |
|
| |||||||||
| PC | 16:0/16:1 | 13.9 ± 1.61 | 12.44 ± 2.13 | 20.37 ± 0.65 | 17.79 ± 0.64 | - | 0.61 | −12% | 0.047 |
|
| |||||||||
| LPE | 18:0/0:0 | 2.78 ± 0.27 | 3.98 ± 0.27 | 3.25 ± 0.2 | 3.79 ± 0.03 | +43% | 0.035 | - | 0.053 |
|
| |||||||||
| PE | Total | 39.78 ± 2.68 | 40.14 ± 3.53 | 49.14 ± 0.85 | 44.01 ± 0.98 | - | 0.94 | −10% | 0.017 |
|
| |||||||||
| 16:0/18:2 | 1.51 ± 0.22 | 1.37 ± 0.34 | 1.91 ± 0.08 | 1.55 ± 0.05 | - | 0.75 | −19% | 0.016 | |
| 16:0/18:1 | 6.47 ± 0.6 | 6.15 ± 0.92 | 8.89 ± 0.18 | 7.48 ± 0.17 | - | 0.78 | −16% | 0.005 | |
| 17:0/17:0 | 0.57 ± 0.05 | 0.53 ± 0.03 | 0.47 ± 0.004 | 0.41 ± 0.01 | - | 0.56 | −13% | 0.019 | |
| 18:1/18:2 | 0.84 ± 0.08 | 0.84 ± 0.1 | 1.03 ± 0.04 | 0.90 ± 0.03 | - | 0.99 | −12% | 0.038 | |
| 18:0/18:2 | 6.31 ± 0.56 | 6.41 ± 0.87 | 7.8 ± 0.22 | 6.98 ± 0.19 | - | 0.93 | −10% | 0.048 | |
| 18:0/18:1 | 12.32 ± 0.68 | 12.36 ± 0.92 | 14.69 ± 0.04 | 13.2 ± 0.23 | - | 0.97 | −10% | 0.0029 | |
| 16:0/22:6 | 0.59 ± 0.06 | 0.58 ± 0.06 | 0.78 ± 0.03 | 0.67 ± 0.02 | - | 0.84 | −14% | 0.045 | |
| 18:1/22:6 | 0.24 ± 0.02 | 0.24 ± 0.02 | 0.26 ± 0.01 | 0.23 ± 0.004 | - | 0.96 | −11% | 0.049 | |
|
| |||||||||
| pPE | 16:0p/18:1 | 0.32 ± 0.027 | 0.33 ± 0.026 | 0.57 ± 0.016 | 0.46 ± 0.009 | - | 0.8 | −19% | 0.0036 |
| 16:0p/20:4 | 0.11 ± 0.014 | 0.13 ± 0.011 | 0.18 ± 0.007 | 0.16 ± 0.001 | - | 0.33 | −11% | 0.030 | |
|
| |||||||||
| PS | 16:0/18:1 | 5.55 ± 0.29 | 5.14 ± 0.58 | 8.10 ± 0.17 | 7.10 ± 0.25 | - | 0.56 | −12% | 0.029 |
|
| |||||||||
| ceramide | Total | 7.33 ± 0.603 | 6.07 ± 0.242 | 8.84 ± 0.362 | 6.39 ± 0.223 | - | 0.12 | −28% | 0.0044 |
|
| |||||||||
| d18:1/14:0 | 0.24 ± 0.022 | 0.18 ± 0.015 | 0.3 ± 0.024 | 0.18 ± 0.005 | - | 0.09 | −38% | 0.0085 | |
| d18:1/16:0 | 1.62 ± 0.081 | 1.39 ± 0.076 | 1.85 ± 0.109 | 1.32 ± 0.039 | - | 0.11 | −29% | 0.01 | |
| d18:1/18:0 | 0.036 ± 0.004 | 0.028 ± 0.002 | 0.043 ± 0.002 | 0.028 ± 0.003 | - | 0.13 | −25% | 0.01 | |
| d18:1/20:0 | 0.027 ± 0.002 | 0.017 ± 0.003 | 0.043 ± 0.004 | 0.029 ± 0.002 | - | 0.061 | −35% | 0.025 | |
| d18:1/22:0 | 0.5 ± 0.055 | 0.39 ± 0.017 | 0.69 ± 0.013 | 0.48 ± 0.044 | - | 0.14 | −32% | 0.01 | |
| d18:1/24:1 | 2.33 ± 0.373 | 1.77 ± 0.226 | 2.56 ± 0.07 | 1.69 ± 0.092 | - | 0.27 | −33% | 0.0017 | |
| d18:1/24:0 | 1.88 ± 0.105 | 1.63 ± 0.109 | 2.31 ± 0.125 | 1.76 ± 0.067 | - | 0.16 | −24% | 0.018 | |
| d18:1/26:0 | 0.039 ± 0.005 | 0.034 ± 0.04 | 0.047 ± 0.001 | 0.037 ± 0.001 | - | 0.58 | −21% | 0.0011 | |
WT SR-B1- and SR-B1-Q445A-expressing cells were cultured in absence (−) or in presence of lipid micelles (PPM) for 15 min. Detergent-resistant membranes (DRM) were isolated through a sucrose gradient and lipid concentrations were determined by LC-MS/MC analysis. Results (mM) are expressed as mean ± SEM from three independent experiments. The species representing more than 10% of the total amount of species analyzed in their lipid subclass are underlined. Statistical changes (variation and p value) are in bold.