Table 2.
List of proteins whose abundance in DRM changes after PPM supply in WT SR-B1- versus SR-B1-Q445A-expressing cells.
| WT SR-B1 | Q445A-SR-B1 | |||||
|---|---|---|---|---|---|---|
|
| ||||||
| fold change after PPM | p value | Uniprot | protein name | function | fold change after PPM | p value |
| 212.49 | 2.34E-05 | P00403 | COX2, Cytochrome C oxydase sub unit 2 | oxidation-reduction | 0.67 | 0.517 |
| 76.88 | 3.5E-07 | Q9UJU6 | DBNL, Drebin-like protein | cell adhesion/junction and cytoskeleton | 1.27 | 0.345 |
| 9.42 | 0.012 | P40227 | TCPZ, T-complex protein 1 subunit zeta | protein interaction/processing | 0.52 | 0.369 |
| 8.42 | 0.007 | P42167 | LAP2B, Lamina-associated polypeptide 2 isoforms b/g | nucleus/transcription/translation | 1.38 | 0.747 |
| 8.17 | 1.01E-05 | P46777 | RL560S, ribosomal protein L5 | protein interaction/processing | 1.09 | 0.715 |
| 7.13 | 0.014 | Q9Y5K8 | VATD, V-type proton ATPase subunit D | ion transport | 0.76 | 0.77 |
| 6.89 | 0.016 | P37059 | DHB2, Estradiol 17-beta-dehydrogenase | steroid, lipid metabolism | 0.85 | 0.864 |
| 6.29 | 1.4E-07 | O43169 | CYB5B, Cytochrome b5 | oxidation-reduction | 1.31 | 0.011 |
| 5.55 | 0.032 | P61978 | HNRPK, Heterogeneous nuclear ribonucleoprotein K | nucleus/transcription/translation | 1.73 | 0.524 |
| 4.10 | 0.009 | O75381 | PEX14, Peroxisomal membrane protein PEX14 | protein interaction/processing | 0.80 | 0.727 |
| 3.90 | 0.004 | P56134 | ATPK, ATP synthase subunit f | oxidation-reduction | 0.52 | 0.071 |
| 3.55 | 0.044 | Q9NRR3 | C42S2, Cdc42 small effector protein 2 | cell adhesion/junction and cytoskeleton | 0.89 | 0.851 |
| 3.36 | 0.011 | O95994 | AGR2, Anterior gradient protein 2 homolog | protein interaction/processing | 0.12 | 0.0006 |
| 3.13 | 0.004 | O43678 | NDUA2, NAH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 2 | oxidation-reduction | 1.09 | 0.801 |
| 3.12 | 0.047 | P50454 | SERPH, Serpin H | cell adhesion/junction and cytoskeleton | 0.46 | 0.175 |
| 3.01 | 0.002 | Q8NBX0 | SCPDL, Saccharopine dehydrogenase-like oxidoreductase | oxidation-reduction | 1.31 | 0.456 |
| 2.87 | 0.035 | Q12907 | LMAN2, Vesicular integral-membrane protein VIP36 | vesicular traficking/autophagy | 1.14 | 0.815 |
| 2.65 | 0.025 | P51114 | FXR1, Fragile X mental retardation syndrome-related protein 1 | nucleus/transcription/translation | 1.80 | 0.123 |
| 2.54 | 0.01 | P40879 | S26A3, Chloride-anion exchanger | ion transport | 0.98 | 0.956 |
| 2.46 | 0.003 | Q9H6A9 | PCX3, Pecanex-like protein 3 | unclassified | 2.36 | 0.0033 |
| 2.28 | 0.017 | P36578 | RL460S, ribosomal protein L4 | protein interaction/processing | 1.40 | 0.296 |
| 2.17 | 0.015 | Q9Y624 | JAM1, Junctional adhesion molecule A | cell adhesion/junction and cytoskeleton | 1.25 | 0.545 |
| 2.13 | 3.22E-06 | P35527 | K1C9, Keratin, type I cytoskeletal 9 | cell adhesion/junction and cytoskeleton | 0.60 | 0.0019 |
| 2.12 | 1.41E-06 | P27824 | CALX, Calnexin | protein interaction/processing | 1.01 | 0.974 |
| 2.06 | 5.95E-05 | Q16864 | VATFV-type proton ATPase subunit F | ion transport | 1.37 | 0.0061 |
|
| ||||||
| 0.50 | 0.031 | P13861 | KAP2, cAMP-dependent protein kinase type II-alpha regulatory subunit | signal transduction | 0.81 | 0.547 |
| 0.49 | 0.002 | Q15363 | TMED2, Transmembrane emp24 domain-containing protein 2 | vesicular traficking/autophagy | 0.66 | 0.0197 |
| 0.47 | 0.0036 | Q9Y2B5 | VP9D1, VPS9 domain-containing protein 1 | ion transport | 1.78 | 0.012 |
| 0.46 | 0.0067 | p07195 | LDHB, L-lactate dehydrogenase B chain | oxidation-reduction | 1.66 | 0.035 |
| 0.43 | 0.002 | Q14974 | IMB, 1Importin subunit beta-1 | protein interaction/processing | 1.16 | 0.394 |
| 0.41 | 0.012 | Q9BV40 | VAMP8, Vesicle-associated membrane protein 8 | vesicular traficking/autophagy | 0.97 | 0.956 |
| 0.41 | 0.044 | Q5T0W9 | FA83B, Protein FAM83B | signal transduction | 0.74 | 0.541 |
| 0.41 | 0.011 | Q8N3R9 | MPP5, MAGUK p55 subfamily member 5 | cell adhesion/junction and cytoskeleton | 0.76 | 0.515 |
| 0.40 | 0.005 | O14662 | STX16, Syntaxin-16 | vesicular traficking/autophagy | 2.88 | 0.002 |
| 0.38 | 0.030 | Q99536 | VAT1, Synaptic vesicule amine transport | oxidation-reduction | 1.02 | 0.974 |
| 0.38 | 0.016 | Q99623 | PHB2, Prohibitin-2 | nucleus/transcription/translation | 1.40 | 0.412 |
| 0.32 | 0.001 | P05362 | ICAM1, Intercellular adhesion molecule 1 | cell adhesion/junction and cytoskeleton | 0.77 | 0.411 |
| 0.29 | 9.3E-05 | Q5T0Z8 | CF132, Uncharacterized protein C6orf132 | unclassified | 1.22 | 0.224 |
| 0.25 | 0.0002 | P08574 | CY1, Ubiquinol-Cytochrome-C Reductase Complex Cytochrome C1 Subunit |
oxidation-reduction | 0.48 | 0.043 |
| 0.17 | 1.92E-06 | P31943 | HNRH1, Heterogeneous nuclear ribonucleoprotein H | nucleus/transcription/translation | 1.41 | 0.022 |
| 0.14 | 0.024 | O60658 | PDE8A, High affinity cAMP-specific and IBMX-insensitive 3′,5′-cyclic phosphodiesterase 8A | signal transduction | 0.92 | 0.950 |
WT SR-B1- and SR-B1-Q445A- expressing cells were cultured in absence (−) or in presence of lipid micelles (PPM) for 15 min. Detergent-resistant membranes (DRM) were isolated through sucrose gradient and protein enrichment was determined by tandem mass spectrometry analysis. Proteins with a fold change higher than 2 in DRM from WT SR-B1-expressing cells after PPM supply are listed. Fold change variation of these proteins in DRM from SR-B1-Q445A-expressing cells are reported. P values <0.05 are in bold. Proteins with similar direction change in their abundance in DRM after PPM in WT SR-B1- and SR-B1-Q445A-expressing cells are highlighted in green. Proteins with opposite variations induced by PPM in DRM in WT SR-B1- versus SR-B1-Q445A expressing cells are highlighted in red. Proteins unchanged in abundance in DRM after PPM supply in SR-B1-Q445A-expressing cells are indicated by a lack of highlighting.