Table 1.

Enzyme kinetics of PDF

S. no	Crude PDF activity	rPDF activity	PDF activity with iron
K M	1.4 mM	1.3 mM	0.1 mM
V max	0.030 mM/mg/min	0.035 mM/mg/min	0.053 mM/mg/min
Specific activity	0.007 mM of NADH/mg/min	0.015 mM of NADH/mg/min	0.069 mM of NADH/mg/min

The PDF activity remains the same in the crude and recombinant fractions; however, it has been increased with iron supplementation